Physiological and Phytochemical Studies on Kaff Maryum (Anastaticahierochuntica L.) plant

Abstract

In present work the effect of red sea water ,mannitol and their interaction on kaff maryum plant was studied .The study included two parts ;the first was the physiological results and the second the phytochemical results. The physiological results The percentage of germination and shoot length were reduced while the root length, fresh weight, number of branches and leaves and leave area were mostly increased in response to salinity especially at the low concentration. Soluble sugars were at high level in the low concentration while the high concentration accumulated polysaccharide. The antioxidant compounds showed high levels in most treatments. Also the antioxidant activity increased by increasing the total phenolics and flavonoid contents. There are some qualitative changes in the protein profile which included the disappearance and appearance of de novo synthysized bands. The phytochemical results Five Compounds were isolated for the first time from A. hierochuntica. The extracts of control C, include flavonoids (1-12) and the five treatments (S1, S2, M, MS1 and MS2) were subjected to HPLC analysis as well as CO-PC with authentic samples using two dimension paper chromatography (2D-PC). Twelve flavonoid compounds were isolated and identified from the control extract of A. hierochuntica (vegetative stage).

Keywords: Anastatica hierochuntica – Salinity – Antioxidant – Physiological - Phytochemical