MEASUREMENT OF 8-ISOPROSTANE IN EXHALED BREATH CONDENSATE OF COPD PATIENTS

Abstract

Chronic obstructive pulmonary disease (COPD), a common preventable and treatable disease, is characterized by airflow limitation that is usually progressive and associated with an enhanced chronic inflammatory response in the airways and the lung to noxious particles or gases. Exacerbations and comorbidities contribute to the overall severeity in individual patients. Severeal attempts have been made therefore to detect and monitor inflammatory changes and mediators using non-invasive methods in COPDpatients. Measurements of exhaled breath condensate (EBC) that can be made at workplace directly and noninvasively, and last but not least repeatedly over short periods of time could be valuableInflammatory mediators in EBC that increase at work in most patients with occupational lung disease can be used as objective evidence to support the diagnosis. Analysis of exhaled breath is rapidly emerging as a novel and non-invasive approach for sampling airway epithelial lining fluid and offers another convenient tool to provide biomarkersone ofthe most studied biomarker in patients with COPD is 8-isoprostane. While levels of 8-isoprostane in EBC have consistently been found to be increased in patients with COPD,the increase in 8-isoprostane levels in EBC was found irrespective of the lung function impairment. Therefore we aimed in our study to measure 8 isoprostane in exhaled breath condensate of COPD patients as a biomarker of oxidative stress and we found that:  Smoking is the most important factor increases the severeity of COPD and the level of 8-isoprostane.  In very severecases we found inX-ray changes in lung size.  Patient's characteristics in our study showedthat age is significant with severeity of the disease as severeity increased with old patients.