Biodegradation of some polycyclic aromatic hydrocarbons polluted soils contaminated with petroleum oil

Abstract

Eight bacterial strains designed as MAM-P1, MAM-P8, MAM-P13 and MAM-P14 (group A) were isolated from soil contaminated with crude petroleum oil from El Zytea-port, Suez Canal, Egypt. While bacterial strains MAM-P22, MAM-P25, MAM-P26 and MAM-P39 (group B) were isolated from soil contaminated with crude petroleum oil from Cairo Oil Refining Company, Mostrod, Qalyubia, Egypt. These bacterial isolates were evaluated for their ability to grow on HMW-PAHs (benz[a]anthracene, benzo[a]pyrene and Pyrene) as a sole carbon and energy source. The ability of eight bacterial isolates to grow on different concentrations (500µM, 750 µM, 1000 µM, 1250 µM and 1500 µM) of HMW-PAHs (benz[a]anthracene, benzo[a]pyrene and Pyrene) was investigated by recording their growth and secretion of extracellular protein at zero time (initial) and after 3, 6, 9, 12, 15, 18 and 21days incubation period. These bacterial isolates also degraded and utilized different concentrations [(525µM, 550µM, 610µM