IMPACT OF SOME HEAT RESISTANCE GENES ON QUANTITATIVE TRAITS IN CHICKEN USING MODERN GENETIC TECHNIQUES
OSAMA KORNAH ABOU-EMERA EMARA;
Abstract
Osama Kornah Abou-Emera Emara. Impact of Some Heat Resistance Genes on Quantitative Traits in Chicken Using Modern Genetics techniques. Unpublished Ph.D. Thesis, Department of Poultry Production, Faculty of Agriculture, Ain Shams University, 2017.
According to morphological appearance of feather coverage, a total number of 368 birds, representing three genotypes (heterozygous naked neck (Nanaff), heterozygous frizzle (nanaFf) and normally feathered (nanaff) genotypes) were classified. At sexual maturity, the chickens were individually housed in wire cages located in semi-closed house. Adult body weight, age at sexual maturity, egg number and egg weight were recorded for each genotype. At 30 weeks of age, egg quality characteristics were examined. Forty birds were randomly assigned (eight birds/genotype) to assess cell mediated immunity through PHA-P injection in wattles. Blood samples were collected from the wing vein. The DNA was purified by successive extraction with phenol:chloroform:isoamyl alcohol (25:24:1) and chloroform:isoamyl alcohol (24:1), respectively. A total of 20 microsatellite markers were selected based on the degree of polymorphism reported in the literature. The PCR amplification was carried out in 25 µL reaction volumes, gels were stained in ethidium bromide and DNA bands were visualized on UV-transilluminator. Data of SSR analyses were scored on the basis of the presence or absence of the amplified products for each primer. The similarity coefficients were then used to construct a dendrogram by Unweighted Pair-Group Method with Arithmetical Average (UPGMA).
According to morphological appearance of feather coverage, a total number of 368 birds, representing three genotypes (heterozygous naked neck (Nanaff), heterozygous frizzle (nanaFf) and normally feathered (nanaff) genotypes) were classified. At sexual maturity, the chickens were individually housed in wire cages located in semi-closed house. Adult body weight, age at sexual maturity, egg number and egg weight were recorded for each genotype. At 30 weeks of age, egg quality characteristics were examined. Forty birds were randomly assigned (eight birds/genotype) to assess cell mediated immunity through PHA-P injection in wattles. Blood samples were collected from the wing vein. The DNA was purified by successive extraction with phenol:chloroform:isoamyl alcohol (25:24:1) and chloroform:isoamyl alcohol (24:1), respectively. A total of 20 microsatellite markers were selected based on the degree of polymorphism reported in the literature. The PCR amplification was carried out in 25 µL reaction volumes, gels were stained in ethidium bromide and DNA bands were visualized on UV-transilluminator. Data of SSR analyses were scored on the basis of the presence or absence of the amplified products for each primer. The similarity coefficients were then used to construct a dendrogram by Unweighted Pair-Group Method with Arithmetical Average (UPGMA).
Other data
| Title | IMPACT OF SOME HEAT RESISTANCE GENES ON QUANTITATIVE TRAITS IN CHICKEN USING MODERN GENETIC TECHNIQUES | Other Titles | تأثير بعض العوامل الوراثية المرتبطة بمقاومة الإجهاد الحراري على الصفات الكمية في الدجاج باستخدام التقنيات الوراثية الحديثة | Authors | OSAMA KORNAH ABOU-EMERA EMARA | Issue Date | 2017 |
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