Obese Egyptian Children: What About Their Gut Flora ?
Ibrahim Akeel El-Sharkawy;
Abstract
The identification of the gut microbiota as an environmental factor that modulates host energy and lipid metabolism has revealed a novel therapeutic target to treat metabolic diseases.
The aim of our study was to identify the predominant gut flora in obese versus non obese Egyptian children and a trial to assess the interrelashionship between type of gut flora and degree of obesity.
This study was conducted on 40 children recruited from the outpatient clinic of Pediatric Hospital, Ain Shams University in the period from April 2012 to November 2012.
First, 20 obese and overweight children were recruited as
Group (I) which subdivided subsequently into subgroup (A) which represented Obese children consisted of 13 children (65%) of cases and subgroup (B) which represented overweight children consisted of 7 children (35%) of cases according to the International Obesity Task Force (IOTF) criteria. Then, 20 non obese children were recruited as Group (II), age and sex matched as a control group.
All Cases and control were subjected to full history taking including dietetic history, clinical examination and Anthropometric measurements.
Using Culture Techniques Stool samples were collected from them and cultured on specific media to cultivate anaerobic gut flora ; Bacteroides, Clostridium, Bifidobacterium and Lactobacillus species.
Children included in the study were 22 males (55%) and 18 females (45%) ranging between 7 and 10 years old and mean age was 8.6+ 1 years
Mean BMI in obese group was 25.7 kg/m2 and in over weight group was 21.5 kg/m2 and in control group was 16.4 kg/m2.
Mean of Caloric intake distribution in obese group was 2436.7 Kcal, in overweight group were 2314.2 Kcal and in control group was 1965 Kcal.
Our study showed increase in Clostridia colony counts in fecal samples of Cases (Obese and Overweight) in comparison with control with high significant statistical difference (P= 0.001) but non significant statistical difference detected between the two subgroups of cases (obese and overweight children) (p>0.05).And non significant statistical difference between cases and control group as regards the other organisms ; Bacteroides, Lactobacilli and Bifidobacteria.
Our study showed a possible correlation between the mode of delivery and childhood obesity, our results showed that 55% of obese and overweight children was born by caesarean section in comparison of only 25% of control group ( P= 0.053 )
Our study showed little but non significant relation between breast feeding and formula feeding and the BMI of the studied groups.Results revealed that 60% of cases were breast fed in comparison of 85% of control (P=0.078)
Our study found significant positive association between early weaning ( before 4 month ) and childhood obesity as 75% of cases were weaned before 4th month in comparison with only 25% of control (p=0.002)
The aim of our study was to identify the predominant gut flora in obese versus non obese Egyptian children and a trial to assess the interrelashionship between type of gut flora and degree of obesity.
This study was conducted on 40 children recruited from the outpatient clinic of Pediatric Hospital, Ain Shams University in the period from April 2012 to November 2012.
First, 20 obese and overweight children were recruited as
Group (I) which subdivided subsequently into subgroup (A) which represented Obese children consisted of 13 children (65%) of cases and subgroup (B) which represented overweight children consisted of 7 children (35%) of cases according to the International Obesity Task Force (IOTF) criteria. Then, 20 non obese children were recruited as Group (II), age and sex matched as a control group.
All Cases and control were subjected to full history taking including dietetic history, clinical examination and Anthropometric measurements.
Using Culture Techniques Stool samples were collected from them and cultured on specific media to cultivate anaerobic gut flora ; Bacteroides, Clostridium, Bifidobacterium and Lactobacillus species.
Children included in the study were 22 males (55%) and 18 females (45%) ranging between 7 and 10 years old and mean age was 8.6+ 1 years
Mean BMI in obese group was 25.7 kg/m2 and in over weight group was 21.5 kg/m2 and in control group was 16.4 kg/m2.
Mean of Caloric intake distribution in obese group was 2436.7 Kcal, in overweight group were 2314.2 Kcal and in control group was 1965 Kcal.
Our study showed increase in Clostridia colony counts in fecal samples of Cases (Obese and Overweight) in comparison with control with high significant statistical difference (P= 0.001) but non significant statistical difference detected between the two subgroups of cases (obese and overweight children) (p>0.05).And non significant statistical difference between cases and control group as regards the other organisms ; Bacteroides, Lactobacilli and Bifidobacteria.
Our study showed a possible correlation between the mode of delivery and childhood obesity, our results showed that 55% of obese and overweight children was born by caesarean section in comparison of only 25% of control group ( P= 0.053 )
Our study showed little but non significant relation between breast feeding and formula feeding and the BMI of the studied groups.Results revealed that 60% of cases were breast fed in comparison of 85% of control (P=0.078)
Our study found significant positive association between early weaning ( before 4 month ) and childhood obesity as 75% of cases were weaned before 4th month in comparison with only 25% of control (p=0.002)
Other data
| Title | Obese Egyptian Children: What About Their Gut Flora ? | Other Titles | الأطفال المصريون البدناء: ماذا عن النبيت الجرثومي المعوي لديهم ؟ | Authors | Ibrahim Akeel El-Sharkawy | Issue Date | 2010 |
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