Novel Spectrofluorimetric Methods for the Assessment of Some Important Industrial Products
Amal Ahmed Mohamed;
Abstract
The study presented in this thesis include development and the combination between some techniques and technologies such as Analytical chemistry, Biochemistry, Photochemistry and Nanotechnology for the determination of some active drugs components (e.g. warfarin sodium) and human enzymes such as alpha amylase .
The thesis is divided into three chapters:
Chapter one :-
Includes a general introduction on lanthanides, optical sensors and other methods used for determination of alpha amylase and warfarin sodium.
Chapter two :-
Describes a new nano-optical sensor Tb3+-ACAC complex doped in sol-gel matrix for assessment of alpha - amylase activity in human saliva and using the alpha amylase as biomarker for the determination of SNS activity in some real saliva samples. The optimal experimental conditions such as solvent, pH and molar ratio corresponding to maximum luminescence intensity were investigated. Under optimal conditions, the luminescence intensity of Tb3+-ACAC doped in sol gel matrix is inversely proportional to the concentration of 2-CNP in range ( 3.0 X 10-4 – 8.0 X 10-9 ) mol L−1, ( r = 0.999 ). The detection limit ( S:N=3) ( LOD) and quantitation limit of detection (LOQ) are (1.2 x 10-9 and 3.6 x 10-9 ) mol L-1 respectively. This method is used for the determination of alpha amylase in human saliva .
Chapter three :-
Describes novel, simple, sensitive and selective spectrofluorimetric method for the determination of warfarin sodium in pharmaceutical tablets, urine and serum samples using optical sensor Eu3+-ACAC . The warfarin sodium can remarkably quench the luminescence intensity of Eu3+-ACAC complex in DMF at pH (6.43) and λex= 395 nm. The quenching of luminescence intensity peak of Eu3+-ACAC complex at λem = 616 nm is proportional to the concentration of warfarin sodium a result that suggested profitable application as a simple optical sensor for warfarin sodium assessment. The dynamic ranges found for the determination of warfarin sodium concentration are (1.6×10-9– 2.1×10-6) mol L-1. The limit of detection (LOD) and quantitation limit of detection (LOQ) are ( 7.50x10-10 and 2.27x 10-9 ) mol L-1 respectively.
The thesis is divided into three chapters:
Chapter one :-
Includes a general introduction on lanthanides, optical sensors and other methods used for determination of alpha amylase and warfarin sodium.
Chapter two :-
Describes a new nano-optical sensor Tb3+-ACAC complex doped in sol-gel matrix for assessment of alpha - amylase activity in human saliva and using the alpha amylase as biomarker for the determination of SNS activity in some real saliva samples. The optimal experimental conditions such as solvent, pH and molar ratio corresponding to maximum luminescence intensity were investigated. Under optimal conditions, the luminescence intensity of Tb3+-ACAC doped in sol gel matrix is inversely proportional to the concentration of 2-CNP in range ( 3.0 X 10-4 – 8.0 X 10-9 ) mol L−1, ( r = 0.999 ). The detection limit ( S:N=3) ( LOD) and quantitation limit of detection (LOQ) are (1.2 x 10-9 and 3.6 x 10-9 ) mol L-1 respectively. This method is used for the determination of alpha amylase in human saliva .
Chapter three :-
Describes novel, simple, sensitive and selective spectrofluorimetric method for the determination of warfarin sodium in pharmaceutical tablets, urine and serum samples using optical sensor Eu3+-ACAC . The warfarin sodium can remarkably quench the luminescence intensity of Eu3+-ACAC complex in DMF at pH (6.43) and λex= 395 nm. The quenching of luminescence intensity peak of Eu3+-ACAC complex at λem = 616 nm is proportional to the concentration of warfarin sodium a result that suggested profitable application as a simple optical sensor for warfarin sodium assessment. The dynamic ranges found for the determination of warfarin sodium concentration are (1.6×10-9– 2.1×10-6) mol L-1. The limit of detection (LOD) and quantitation limit of detection (LOQ) are ( 7.50x10-10 and 2.27x 10-9 ) mol L-1 respectively.
Other data
| Title | Novel Spectrofluorimetric Methods for the Assessment of Some Important Industrial Products | Other Titles | طرق طيفية مبتكرة لتقدير بعض المنتجات الهامة فى الصناعة | Authors | Amal Ahmed Mohamed | Issue Date | 2015 |
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