Kinetic studies for some enzyme catalyzed reactions

Abstract

In this study, we have chosen for study the Horse Radish Peroxidase conjugated to a specific mouse antibody against human hepatitis B virus. This conjugated Horse radish peroxisdase (CHRP) is commonly used as an important component in enzyme Immunoassays (EIA) for detection of hepatitis B (surface) antigen in human serum or plasma.[34] We have studied the characteristics of this CHRP enzymatic reaction, factors affecting its catalytic behavior, and how some substances could inhibit its action, determination of some important catalytic and thermodynamic parameters. We can classify our study into three main Parts: Part 1: Introduction In this part, important information have been mentioned about the enzyme properties generally, and specially about the native Horse radish peroxidase (HRP). Moreover, the introduction includes literature survey on the studies that had been done on Horse radish peroxidase and also the aim of the work.