Advanced studies on Enterococcus species recovered from poultry

Abstract

Out of 150 samples 38 chicken samples (7 brains, 23 livers and 8 hearts), 41 pigeon samples (6 livers and 35 swabs), 41 duck samples (15 brains, 20 livers and 6 hearts) and 30 human samples (30 Urine samples). The prevalence of Enterococcus isolates were 79 %, 100%, 85.4% and 66.7% in the chickens, pigeons, ducks and human samples respectively. Enterococcus spp. showed gelatin hydrolysis activity positive in 50% of Enterococcus spp. recovered from chicken, While was positive in a percentage of 34.1% , 25% and 11.4% for Enterococcus spp. recovered from pigeons, human and ducks respectively. Enterococcus spp. isolated from pigeon had the highest casein hydrolysis activity 61% followed by Enterococcus spp. isolated from chicken 53.3% then Enterococcus spp. isolated from ducks 42.9% and the lowest casein hydrolysis activity was Enterococcus spp. isolated from human source 10%. The Enterococcus spp. results for affinity percentage from Chicken were 70% and 30%, Pigeon 61% and 39%, Ducks 68.6% and 31.4% and Human 95% and 5% as moderate and strong biofilm forming capability respectively. All Enterococcus isolates supernatant fluid showed cytopathic effect on the Vero cell line (100%).The prevalence of the total γ-hemolysis activity of Enterococcus spp. reached 66.7% as well as, the β-hemolysis activity and α– hemolysis reached 29.4% and 4% respectively.