Effect of germanium complexes on hepatocellular carcinoma cell line
Iman Ismail Taha;
Abstract
Background and aim: Organogermanium (Ge) complexes are used as dietary supplement. Studies revealed that Ge complexes exhibited analgesic, anti-inflammatory, antioxidant, immune-modulating, antiviral, and anticancer activity. Ascorbic acid is an essential vitamin for human body it is well known with its antioxidant activity. High doses of ascorbic acid showed anticancer activity in different cancer cells. The aim was to determine the antitumor effect of organogermanium complexes, in vitro and in vivo.
Methods: The effect of 12 Ge complexes was tested on HEPG2 cell line, the inhibitory concentration 50 (IC50%) was calculated for each of the complexes. The best 4 complexes, with least IC50, were used for further studies. For the in vivo experiments, 2 complexes were chosen and tested on mice bearing Ehrlich ascites carcinoma. Their effect on EAC and the immune response interleukin 2 (IL-2) and interleukin 6 (IL-6) were compared to that of doxorubicin.
Results: The results were compared to doxorubicin as positive control. The four complexes were tested alone or combined with high dose of ascorbic acid. The results showed a strong antitumor activity of the Ge-complexes. IC50 values were less than that of doxorubicin, indicating a stronger inhibition of tumor growth. Furthermore, a significant decrease was observed in the proliferation marker Ki67, and the anti-apoptotic marker bcl-2, together with an increase in the apoptotic marker caspase3. The combination with ascorbic acid increased the activity of some complexes acting in synergetic way. The in vivo results revealed an increase in the survival rate of mice treated with the complexes than the untreated group. When compared to doxorubicin; the positive control, the complexes showed a comparable effect on mice survival, ascites volume, and number of dead cells; whether alone or combined with vitamin C. Furthermore, a decrease in IL-2 and IL-6 levels was observed, indicating a decrease in cancer progression.
Methods: The effect of 12 Ge complexes was tested on HEPG2 cell line, the inhibitory concentration 50 (IC50%) was calculated for each of the complexes. The best 4 complexes, with least IC50, were used for further studies. For the in vivo experiments, 2 complexes were chosen and tested on mice bearing Ehrlich ascites carcinoma. Their effect on EAC and the immune response interleukin 2 (IL-2) and interleukin 6 (IL-6) were compared to that of doxorubicin.
Results: The results were compared to doxorubicin as positive control. The four complexes were tested alone or combined with high dose of ascorbic acid. The results showed a strong antitumor activity of the Ge-complexes. IC50 values were less than that of doxorubicin, indicating a stronger inhibition of tumor growth. Furthermore, a significant decrease was observed in the proliferation marker Ki67, and the anti-apoptotic marker bcl-2, together with an increase in the apoptotic marker caspase3. The combination with ascorbic acid increased the activity of some complexes acting in synergetic way. The in vivo results revealed an increase in the survival rate of mice treated with the complexes than the untreated group. When compared to doxorubicin; the positive control, the complexes showed a comparable effect on mice survival, ascites volume, and number of dead cells; whether alone or combined with vitamin C. Furthermore, a decrease in IL-2 and IL-6 levels was observed, indicating a decrease in cancer progression.
Other data
| Title | Effect of germanium complexes on hepatocellular carcinoma cell line | Other Titles | تاثير متراكبات الجرمانيوم علي خط خلايا سرطان الكبد الخلوي | Authors | Iman Ismail Taha | Issue Date | 2019 |
Attached Files
| File | Size | Format | |
|---|---|---|---|
| CC3869.pdf | 249.74 kB | Adobe PDF | View/Open |
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