Evaluation of Color Stability, Water Sorption, Solubility and Bond Durability of an Adhesive after Adding Epigallocatechin-Gallate in Two Different Concentrations
Nadia Taha El-Ansary;
Abstract
Our study aimed to investigate the effect of adding EGCG in two different concentrations 100µg/ml 200µg/ml on the color stability,
water sorption and bond strength of an etch and rinse dental adhesive to dentin.
According to literature Matrix metalloproteinases became active after acid etching the dentin, they degrade the collagen fibers which affects the bond strength to dentin and the durability of the resin dentin bond. EGCG is the main flavonoid of green tea and is known to preserve the bond strength to dentin by acting as collagen cross linker and MMps inhibitor.
EGCG was applied to the adhesive in two different concentrations forming EGCG/adhesive mix with concentration of 100µg/ml and 200µg/ml.
Color stability testing of EGCG/adhesive mix in both concentrations and the control group was done by applying an adhesive layer to IPS emax discs.
The color of each disc was measured using Agilent Cary 5000 spectrophotometer (Santa Clara, California, United States) before applying the adhesive, 24 hours, 6 month and 1 year after applying it. During this period the discs were stored in distilled water.
Water sorption and solubility testing of EGCG/adhesive mix in both concentrations and the control group was done by weighing the samples before and after being placed in distilled water for 7 days following the 4049 ISO standards.
Infrared spectroscopy of the distilled water before and after removing the discs from it was done using Vertex 70 FTIR spectrometer (Burker, Germany)
Microtensile bond strength testing of the two concentrations of EGCG in the adhesive was done using universal testing machine (Instron, MA, USA) and compared to the control group. Load applied was 500 N at cross head speed of 0.5 mm/min, until debonding of the specimen occurred. Bond strength was calculated by Bluehill lite software and mode of failure was detected by scanning electron microscope.
water sorption and bond strength of an etch and rinse dental adhesive to dentin.
According to literature Matrix metalloproteinases became active after acid etching the dentin, they degrade the collagen fibers which affects the bond strength to dentin and the durability of the resin dentin bond. EGCG is the main flavonoid of green tea and is known to preserve the bond strength to dentin by acting as collagen cross linker and MMps inhibitor.
EGCG was applied to the adhesive in two different concentrations forming EGCG/adhesive mix with concentration of 100µg/ml and 200µg/ml.
Color stability testing of EGCG/adhesive mix in both concentrations and the control group was done by applying an adhesive layer to IPS emax discs.
The color of each disc was measured using Agilent Cary 5000 spectrophotometer (Santa Clara, California, United States) before applying the adhesive, 24 hours, 6 month and 1 year after applying it. During this period the discs were stored in distilled water.
Water sorption and solubility testing of EGCG/adhesive mix in both concentrations and the control group was done by weighing the samples before and after being placed in distilled water for 7 days following the 4049 ISO standards.
Infrared spectroscopy of the distilled water before and after removing the discs from it was done using Vertex 70 FTIR spectrometer (Burker, Germany)
Microtensile bond strength testing of the two concentrations of EGCG in the adhesive was done using universal testing machine (Instron, MA, USA) and compared to the control group. Load applied was 500 N at cross head speed of 0.5 mm/min, until debonding of the specimen occurred. Bond strength was calculated by Bluehill lite software and mode of failure was detected by scanning electron microscope.
Other data
| Title | Evaluation of Color Stability, Water Sorption, Solubility and Bond Durability of an Adhesive after Adding Epigallocatechin-Gallate in Two Different Concentrations | Other Titles | تقييم الثبات اللوني و امتصاص الماء و دوامية الرابطة للاصق المعتمد علي التخريش و الشطف بعد إضافة خلاصة الشاي الأخضر بتركيزات مختلفة | Authors | Nadia Taha El-Ansary | Issue Date | 2019 |
Attached Files
| File | Size | Format | |
|---|---|---|---|
| cc1249.pdf | 664.98 kB | Adobe PDF | View/Open |
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