Impact of dry orange (Citrus sinensis) peels powder on Bisphenol A induced-hepatic and splenic toxicity in rats

Abstract

This study was conducted to investigate the protective effect of orange peels powder (OPP) dietary supplementation on rats orally administered BPA for 4 weeks. Data revealed that each 100g of OPP contain 5 g protein, 5.61 g fat, 65.67 g carbohydrate, 2.9 g ash, 8.5g moisture and 12.22 g fiber. OPP ethanolic extract had been analyzed for its total phenols and flavonoids content. The results showed that, total phenolic content was 567.93 mg as gallic acid equivalent (GAE), while flavonoidswere 162.12 mg as quercetin equivalent (QE)/per 100 g orange peel powder. In this study, number of 60 male albino rats weighing 130±10g were divided into 6 groups, 10 rats for each as follows: Group 1: (negative control): Rats were fed on balanced diet and received corn oil. Group 2: (positive control or BPA group): Rats were fed on balanced diet + orally administered BPA suspended in corn oil (350mg/kg b.w. twiceweekly). Group 3:Rats were fed on balanced diet supplemented with OPP (12.5g/kg diet) + BPA. Group 4: Rats were fed on balanced diet supplemented with OPP (25g/kg diet) + BPA. Group 5: Rats were fed on balanced diet supplemented with OPP (50/kg diet) + BPA.