Biological Activity of Polymer Bound Bacteriogenic Nanoparticles

Abstract

Green biosynthesis of silver and selenium nanoparticles using environmentally friendly reducing and capping agents is a recent target approached by many researches. Microorganisms are considered cost effective and benign alternative to chemical and physical methods for nanoparticles synthesis. In this work, soil samples were collected along Ismailia Canal bay. Among 40 bacterial isolates, only 4 isolates (Ism 1, Ism 2, Ism 26 and Ism 37), showed the ability to synthesize AgNPs and SeNPs. UV-Vis spectroscopy showed characteristic peaks for nanoparticles. Bacterial isolates showed AgNPs characteristic peaks ranged between 400 and 450 nm, and for SeNPs specific peaks ranged between 200 and 300 nm. DLS analysis was carried out to determine the size of nanoparticles. The results showed that Ism 2 and Ism 26 synthesized the smallest SeNPs and AgNPs ranged from 50 to 120 nm and 7 to 20 nm, respectively. The 2 bacterial isolates were identified by 16S rRNA gene as Bacillus tropicus Ism 2 (MK332444) and Enterobacter cloacae (KP988024) with blast identity 99% for both.