Evaluation of the Therapeutic Potential of Bone Marrow Derived-Mesenchymal Stem Cells in an Experimental Model of Lung Fibrosis

Abstract

The healing potential of MSCs has been demonstrated in numerous lung injury models, however, whether MSCs could be effective for alleviating PF induced by AD remains largely unknown. Additionally, the time period required for MSCs to accomplish maximal mitigative efficacy and the mechanisms by which MSCs engraftment ameliorates lung injury have not been clearly explored. Moreover, the effectiveness of CM derived form MSCs in the moderation of PF remains controversial and no prior study has investigated the restorative indications of MSCs-derived CM in a rat model of PF induced by AD.

We aimed to evaluate the potential curative influence of BM-MSCs, in comparison to CM, on PF induced by AD in rats.

To achieve this goal, BM-MSCs were isolated from adult male Wistar rats, cultured, passaged, characterized and labeled. Also, CM from cultured BM-MSCs were collected and concentrated. The experimental design was constructed as follows: sixty-four adult male Wistar rats were randomly divided into