Study on inhibition of Nanobacteria forming kidney stones by antibiotics, medicinal plants and gamma radiation
Nora Fayez Hassan Soliman Othman;
Abstract
Abstract
Nanobacteria (NB) are considered to contribute to many calcifying diseases including kidney stones which represent a common problem with inadequate prevention exist. Herein, 32 nanobacteria were isolated from 54 kidney stones collected from patients with active urolithiasis from different medical labs. Detection of nanobacteria was carried out using spectrophotometer, macroscopic and microscopic characteristics, as well as scanning electron microscopy (SEM) with Energy-dispersive X-ray spectroscopy (EDX) as well as transmission electron microscope (TEM) techniques and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE).
The results revealed that out of the collected samples, 32 samples were positive for nanobacterial infection, they were Gram negative cocci and coccobacilli, their size ranged from 375 to 600 nm. Results indicated that all isolates were strong biofilm producers. The most biofilm producer isolate was identified by PCR technique using genomic DNA as a template, the result indicated that the presence of a DNA band of about 1400 base pair length.
The susceptibility of the most potent biofilm producing nanobacterial isolates towards some selected antibiotics was evaluated. The result revealed that streptomycin, trimethoprim/sulfamethoxazole and doxycycline had antinanobacterial activity. Decontamination of certain medicinal plants by Gamma irradiation was carried out. The effect of medicinal plant extracts and essential oils on growth of the strongest nanobacterial biofilm producers was tested. Also, their MICs and sub MICs were evaluated and their effect on biofilm production was studied.
Effect of combination treatment between most potent agents was tested on biofilm production, protein profile, release of 260 nm absorbing material, protein content and ultrastructure of the strongest biofilm producers. The findings of this study evidenced that combination treatment between doxycycline (DO) and aqueous extract of irradiated khella exhibited significant reduction in biofilm formation ability of the strongest producers nanobes. Also affect dramatically the strength of its cell membrane and ultrastructure.
Finally, In vivo study of nanobacteria and its treatment by most potent agents was evaluated. Renal function tests were measured, also histological examination and oxidative stress parameters were estimated in kidney tissues. It could be concluded that the combination of DO and aqueous extract of irradiated khella had anti - nephrotoxic effect against nanobacteria induced renal toxicity.
Key Words:
Nanobacteria, biofilm formation, medicinal plant extracts, γ- irradiation and SEM and TEM investigation and in vivo study
Nanobacteria (NB) are considered to contribute to many calcifying diseases including kidney stones which represent a common problem with inadequate prevention exist. Herein, 32 nanobacteria were isolated from 54 kidney stones collected from patients with active urolithiasis from different medical labs. Detection of nanobacteria was carried out using spectrophotometer, macroscopic and microscopic characteristics, as well as scanning electron microscopy (SEM) with Energy-dispersive X-ray spectroscopy (EDX) as well as transmission electron microscope (TEM) techniques and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE).
The results revealed that out of the collected samples, 32 samples were positive for nanobacterial infection, they were Gram negative cocci and coccobacilli, their size ranged from 375 to 600 nm. Results indicated that all isolates were strong biofilm producers. The most biofilm producer isolate was identified by PCR technique using genomic DNA as a template, the result indicated that the presence of a DNA band of about 1400 base pair length.
The susceptibility of the most potent biofilm producing nanobacterial isolates towards some selected antibiotics was evaluated. The result revealed that streptomycin, trimethoprim/sulfamethoxazole and doxycycline had antinanobacterial activity. Decontamination of certain medicinal plants by Gamma irradiation was carried out. The effect of medicinal plant extracts and essential oils on growth of the strongest nanobacterial biofilm producers was tested. Also, their MICs and sub MICs were evaluated and their effect on biofilm production was studied.
Effect of combination treatment between most potent agents was tested on biofilm production, protein profile, release of 260 nm absorbing material, protein content and ultrastructure of the strongest biofilm producers. The findings of this study evidenced that combination treatment between doxycycline (DO) and aqueous extract of irradiated khella exhibited significant reduction in biofilm formation ability of the strongest producers nanobes. Also affect dramatically the strength of its cell membrane and ultrastructure.
Finally, In vivo study of nanobacteria and its treatment by most potent agents was evaluated. Renal function tests were measured, also histological examination and oxidative stress parameters were estimated in kidney tissues. It could be concluded that the combination of DO and aqueous extract of irradiated khella had anti - nephrotoxic effect against nanobacteria induced renal toxicity.
Key Words:
Nanobacteria, biofilm formation, medicinal plant extracts, γ- irradiation and SEM and TEM investigation and in vivo study
Other data
| Title | Study on inhibition of Nanobacteria forming kidney stones by antibiotics, medicinal plants and gamma radiation | Other Titles | دراسة على تثبيط النانوبكتريا التى تسهم فى تكوين حصوات الكلى بواسطة المضادات الحيوية ، النباتات الطبية واشعة جاما | Authors | Nora Fayez Hassan Soliman Othman | Issue Date | 2022 |
Attached Files
| File | Size | Format | |
|---|---|---|---|
| BB13774.pdf | 1.18 MB | Adobe PDF | View/Open |
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