Association Between GLIS3 rs7020673 Polymorphism and Type 2 Diabetes Mellitus among Egyptian Population

Abstract

SUMMARY AND CONCLUSION E nvironmental and genetic variations are key risk factors for T2DM having an important role in the disease pathogenesis. GLIS3 protein is a member of the GLIS subfamily of Krüppel-like zinc finger transcription factors that functions as an activator or repressor of gene expression. GLIS3 plays a critical role in pancreatic cell lineage specification, particularly in the development of mature beta cells. GLIS3 is also important to beta cell mass maintenance and regulation of insulin expression in adults. GWAS in humans have have shown that GLIS3 gene polymorphism may confer risk to T2DM. The present study aimed to investigate the potential association between the GLIS3 (rs7020673) gene polymorphism and T2DM and its impact on glycemic control among Egyptian population. The present study was conducted on 100 T2DM patients (Group I) who were recruited from the Endocrinology Clinic in Ain Shams University Hospitals. They were 49 males and 51 females with a mean age of 59.2 ± 8.9 years. In addition, the study included 100 non-diabetic apparently healthy subjects who were age- and sex- matched with Group I and serving as healthy controls (Group II). Detection of the polymorphism GLIS3 rs7020673 (C>G) by real-time PCR was done for both patient and control groups. In addition, validation assay of random samples of the genotyping results was done by the Sanger sequencing method.