Evaluation of Long Non Coding RNA (CCAT1) as a Prognostic Biomarker in Acute Myeloid Leukemia

Abstract

Long non-coding RNA (CCAT-1) and micro RNA (miRNA-155a) are in a tight relation with different types of cancers as well as in AML. However, the prognostic value of CCAT-1/miR-155a pathway in AML has not been investigated. The purpose of this study is to investigate the correlation of CCAT-1 expression with clinic-pathological features and the clinical prognosis of the AML patients. Also to find out the association between CCAT-1 expression and expression of miRNA-155a as a targeting gene for CCAT-1 and to improve understanding of the roles and the clinic implications of CCAT-1 in the development and progression of AML. This current study was conducted at (Internal Medicine Hospitals, Ain Shams University, Department of Hematology). It was performed on 70 individuals categorized as 50 AML patients and 20 healthy control groups. Peripheral blood samples were collected for molecular analysis of miR-155a, Lnc-CCAT-1 extraction and expression using Real time PCR by SYBR green kit. In AML group, miR-155 expression was highly significant in clinical response with (P≤ 0.004) and was slightly significant in cytogenetic abnormality, BM blasts; MRD with (P≤ 0.05, P≤0.03, P≤0.02)