Detection of Colistin Resistant Gram Negative Bacilli in Intensive Care Unit Patients Admitted to Ain Shams University Hospitals

Abstract

Antimicrobial resistance in Gram-ve bacteria (GNB), especially those of the Enterobacteriaceae family, has become a leading cause of morbidity and mortality and a serious public health concern all over the world. With the spread of highly resistant bacteria such as carbapenemase-producing Enterobacteriaceae, and multi-drug-resistant P. aeruginosa and A. baumannii, colistin has increasingly been used as a last resort agent. Therefore the development of colistin resistance in multidrug-resistant bacteria is an important clinical and public health issue, the emergence of colistin resistance has been detected worldwide in recent years after the emergence of transferable colistin resistance gene mcr-1. The aim of this study was to detect colistin resistant Gram negative bacilli by ComASPTM Colistin (formerly SensiTest™ Colistin) in ICU patients admitted to Ain Shams university hospitals and to screen the presence of mcr-1 gene by PCR in colistin resistant isolates.