Role of Vitamin D Receptor Polymorphism in Nephropathy of Type II Diabetes Mellitus patients

Abstract

The impact of several environmental and genetic factors on diabetes and its complications is well documented but there is an urgent need to understand more about genetic risk factors associated with this disease. Diabetic nephropathy (DN) is one of the most common complications of diabetes mellitus (DM) and some genetic factors may influence susceptibility to nephropathy despite DM is controlled or not. This study is designed to assess the possible association of Taq I polymorphism of vitamin D receptor (VDR) with T2DM and DN in Egyptian patients taking in consideration some biochemical parameters and risk factors. A cross-sectional study enrolled 90 unrelated subjects who divided into 3 groups group 1 included 30 control subjects, group 2 included 30 T2DM patients and group 3 included T2DM patients with DN. samples were collected from the outpatient Clinics of the Internal Medicine Department in El- Kasr Al-Aini hospital, Cairo University, Different biochemical parameters were measured and PCR–RFLP analysis was performed to examine Taq I polymorphism of VDR using Taq I restriction enzyme. Results: There was no statistical significant difference observed in genotypic and allelic distribution of the Taq I polymorphism in T2DM and DN patients in comparison to control subjects. In conclusion: The data of this study suggest that VDR gene polymorphism (Taq I) genotype is not associated with the risk of T2DM and the development of DN as well.