Genetic fingerprint of some Alhagi graecorum populations based on DNA polymorphism

Abstract

The present investigation was conducted to study the genetic diversity among twenty-five Egyptian Alhagi graecorum genotypes collected from five locations (El-Dakhla Oasis, Ain shams university, Wadi El-Rayan, Qarun lake and Siwa Oasis) using individual- and bulked samples-based approaches. Eight ISSR primers revealed a high percentage of polymorphism (84.16%) across all the studied genotypes and (78.35%) among the five populations, while SCoT primers produced 73.57% (ten primers) and 65.38% (nine primers), respectively. Also, average polymorphism information content (PIC) for amplified DNA of individual samples was 0.860 for SCoT and 0.875 for ISSR. The genetic diversity analysis for Alhagi graecorum revealed similar dendrograms with some slight variations. The results of UPGMA cluster analysis and PCoA grouped the twenty-five Alhagi genotypes according to their geographical location. The study also included sequencing for selected unique fragments from SCoT and ISSR profiles (bulked-DNA samples). The obtained results demonstrate that ISSR was better than SCoT to detect genetic diversity among Alhagi graecorum genotypes.