Effect of Adipose Tissue Derived Mesenchymal Stem Cells versus Metformin on Age-Related Changes of the Cerebellum of Albino Rat (Histological and Immune-Histochemical Study)

Abstract

ging process is a driving force behind the age associated diseases. The CNS, including the cerebellum, is affected by the aging process. This study aimed to compare between the possible role of ADMSCs and metformin on age-related structural changes of the cerebellum in female albino rats. Fifty-five female albino rats of different ages (from 4 to 28 months) were used in this study. Their weights ranged from 200 to 300 grams. They were divided into three groups according to their ages: Group I (Adult rats): included 15 rats. Their ages ranged from 4-6 months. They were further subdivided into three equal subgroups: - Subgroup Ia: were left without any interference (negative control). - Subgroup Ib: were given a single intravenous injection of equivalent volume 1ml of Phosphate buffered saline (PBS) in tail vein. - Subgroup Ic: were given oral metformin daily in a dose of 300 mg/kg body weight/day, dissolved in 1ml of distilled water for four weeks.