Detection of five different periodontopathic bacteria in Egyptian and Yemeni periodontitis patients by using Real-Time PCR (Case Control study)

Abstract

Periodontitis is an inflammatory disease involving the destruction of both soft and hard tissue in the periodontal region. Many factors can influence its etiology, but specific Gram-negative anerobic bacterial species are required for its development in subgingival plaque as; P. gingivalis, T. forsythia, T. denticola, P. intermedia, and A.a. For the identification of periodontal microorganisms, different techniques have been used such as culture technique, DNA-DNA hybridization, immunological and immunoenzymatic analyses. Nonetheless, these techniques display different limitations. However, real time PCR method is a most precise, efficient, and rapid method for the detection, identification, and quantification of periodontal microorganisms. The purpose of the present study was to investigate the bacterial levels of P. gingivalis, T. forsythia, T. denticola, P. intermedia, and A.a in subgingival plaque in patients with grade B periodontitis and healthy individuals in a group of Egyptian and Yemeni participants. The present study was conducted on (80) participants, (40) participants were from the Egyptian population, and the other (40) participants were from the Yemeni population. The Egyptian participants were divided into two groups, (20) participants with periodontitis and (20) healthy controls. The Yemeni participants were divided into two groups, (20) participants with periodontitis and (20) healthy controls.