Invetigating the significance of nucleotides dietary fortification in broiler diet to cope with the diffrtrnt stressors
Nader Farouk Kamel Osman;
Abstract
Two experiments conducted to determine the effects of exogenous dietary nucleotides supplementation on broiler performance, gut health parameters, and immunity.
Experimental design
Experiment-I) The trial has been conducted to evaluate the efficiency of dietary nucleotides-supplementation to broiler chickens in alleviating the intestinal Clostridium perfringens levels and its adverse effect on gut and growth performance parameters. In this study, 270 one-day-old mixed broiler chicks (Cobb 500) randomly divided into 6 treatment groups with 3 replicates of 15 chicks each. Treatment 1 (CX), a negative control group fed corn-soybean basal diet without added nucleotides. Treatment 2 (CN 0.05) and treatment 3 (CN 0.1), consisted of chicks fed the basal diet with the addition of nucleotides on top at two levels (0.05 and 0.1 %) respectively. Treatment 4 (PX), treatment 5 (PN 0.05), and treatment 6 (PN 0.1) consisted of chicks that were challenged with C. perfringens inoculum (~ 4×108 CFU/ml) on d 14, 15, 16 and 17of the experiment and fed diets similar to treatments 1, 2, and 3 respectively. The trial continued for 35 days. At the end of the experiment, intestinal C.perfringens counts, microscopic lesion scores, intestinal histomorphology, intestinal barriers (occludin and mucin mRNA expression), growth parameters were determined.
Experiment-II) The trial conducted to evaluate the efficiency of dietary nucleotides supplementation to overcome the stressful effect of high stocking on performance, gut health parameters, digestive enzymes, and immunity of broiler chickens. In this study, a total of 315 one-day-old mixed broiler chicks (Cobb 500) were randomly assigned to 6 treatment groups with 3 replicates (the numer of chicks/replicate differe according the required stocking density). A 2×3 factorial design with two stocking densities, low stocking density (LSD) 0.073 m2/2.2kg bird (15 chick/ replicate) and high stocking density (HSD) 0.05 m2/2.2 kg bird (20 chick/ replicate), and 3 levels of dietary NP (0,
Experimental design
Experiment-I) The trial has been conducted to evaluate the efficiency of dietary nucleotides-supplementation to broiler chickens in alleviating the intestinal Clostridium perfringens levels and its adverse effect on gut and growth performance parameters. In this study, 270 one-day-old mixed broiler chicks (Cobb 500) randomly divided into 6 treatment groups with 3 replicates of 15 chicks each. Treatment 1 (CX), a negative control group fed corn-soybean basal diet without added nucleotides. Treatment 2 (CN 0.05) and treatment 3 (CN 0.1), consisted of chicks fed the basal diet with the addition of nucleotides on top at two levels (0.05 and 0.1 %) respectively. Treatment 4 (PX), treatment 5 (PN 0.05), and treatment 6 (PN 0.1) consisted of chicks that were challenged with C. perfringens inoculum (~ 4×108 CFU/ml) on d 14, 15, 16 and 17of the experiment and fed diets similar to treatments 1, 2, and 3 respectively. The trial continued for 35 days. At the end of the experiment, intestinal C.perfringens counts, microscopic lesion scores, intestinal histomorphology, intestinal barriers (occludin and mucin mRNA expression), growth parameters were determined.
Experiment-II) The trial conducted to evaluate the efficiency of dietary nucleotides supplementation to overcome the stressful effect of high stocking on performance, gut health parameters, digestive enzymes, and immunity of broiler chickens. In this study, a total of 315 one-day-old mixed broiler chicks (Cobb 500) were randomly assigned to 6 treatment groups with 3 replicates (the numer of chicks/replicate differe according the required stocking density). A 2×3 factorial design with two stocking densities, low stocking density (LSD) 0.073 m2/2.2kg bird (15 chick/ replicate) and high stocking density (HSD) 0.05 m2/2.2 kg bird (20 chick/ replicate), and 3 levels of dietary NP (0,
Other data
| Title | Invetigating the significance of nucleotides dietary fortification in broiler diet to cope with the diffrtrnt stressors | Other Titles | دراسات عن تأثير الإضافه الغذائيه للنيوكليوتيدات على دجاج التسمين تحت التحديات المختلفه | Authors | Nader Farouk Kamel Osman | Issue Date | 2020 |
Attached Files
| File | Size | Format | |
|---|---|---|---|
| BB2061.pdf | 1.05 MB | Adobe PDF | View/Open |
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