PREPARATION OF A VACCINE TO CONTROL THE DEVELOPMENT OF FASCIOLA GIGANTICA IN SHEEP
Noha Ahmed Mahana Osman;
Abstract
Fascioliasis caused by trematodes of the genus
Fasciola, is one of the most important diseases of farmed
ruminants, beside its high prevalence in human in
er
temperate and tropical zones. The appearance of F. gigantica population that IS resistant to common flukicidal drugs means that new methods of treatment will soon be required. The future prospect of anti-liver fluke vaccines requires identification of prime fluke targets for which new vaccine can be developed.
In the present study Sup.2 adult F. gigantica worm antigens were first identified by SOS-PAGE. The electrophoresis migration pattern of Sup.2 antigens in polyacrylamide gel showed 19 bands by
Coomassi blue stain, in the 106-1 o kDa regions. 60, 32 and 28 kDa
were selected because of their immunogenicity and their significant protection in mice. These bands were eluted and used as a vaccine against sheep fascioliasis.
Sixteen sheep were divided into 4 groups: control, D60, G32 and G28. D60, G32 and G28 were vaccinated 6 times with the eluted protein fractions of m 60, 32 and 28 kDa, respectively. After that all sheep groups were bled and the sera were separated to be used for the following experiment.
Fasciola, is one of the most important diseases of farmed
ruminants, beside its high prevalence in human in
er
temperate and tropical zones. The appearance of F. gigantica population that IS resistant to common flukicidal drugs means that new methods of treatment will soon be required. The future prospect of anti-liver fluke vaccines requires identification of prime fluke targets for which new vaccine can be developed.
In the present study Sup.2 adult F. gigantica worm antigens were first identified by SOS-PAGE. The electrophoresis migration pattern of Sup.2 antigens in polyacrylamide gel showed 19 bands by
Coomassi blue stain, in the 106-1 o kDa regions. 60, 32 and 28 kDa
were selected because of their immunogenicity and their significant protection in mice. These bands were eluted and used as a vaccine against sheep fascioliasis.
Sixteen sheep were divided into 4 groups: control, D60, G32 and G28. D60, G32 and G28 were vaccinated 6 times with the eluted protein fractions of m 60, 32 and 28 kDa, respectively. After that all sheep groups were bled and the sera were separated to be used for the following experiment.
Other data
| Title | PREPARATION OF A VACCINE TO CONTROL THE DEVELOPMENT OF FASCIOLA GIGANTICA IN SHEEP | Other Titles | تحضير لقاح يؤثر على تطور الدودة الكبدية (فاشيولا جيجنتيكا) فى الاغنام | Authors | Noha Ahmed Mahana Osman | Issue Date | 2002 |
Attached Files
| File | Size | Format | |
|---|---|---|---|
| B16853.pdf | 2.52 MB | Adobe PDF | View/Open |
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